Requirement for translationally regulated candidate genes during plant NB-LRR- mediated defense responses
T. BARFF (1), L. Meteigner (2), M. Cohen (2), M. El Oirdi (2), K. Yoshioka (3), P. Moffett (2) (1) Université de sherbrooke, Canada; (2) Université de Sherbrooke, Canada; (3) University of Toronto, Canada

Plants possess multiple defense mechanism, including the recognition of pathogen-encoded effectors by the NB-LRR proteins, which in turn leads to the induction of Effector-Triggered Immunity (ETI). Although numerous studies have focused on the recognition and activation of NB-LRR protein, much remains to be understood about downstream signaling in ETI. While the transcriptional regulation following NB-LRR activation has been well-characterized, post transcriptional control during the defense response has not. In a previous study we analysed the translatome upon activation of the NB-LRR protein, RPM1 by TRAPseq. This analysis identified number of candidate genes that are differentially regulated at the translational level upon NB-LRR activation. To determine the importance of translationally regulated genes in plant defense, we infected multiple A. thaliana knock out lines of selected candidate genes with Pseudomonas syringae pv tomato (Pto) DC 3000 AvrRpm1, Pto DC 3000 AvrRps2 and Hyaloperonospora arabidopsidis (Hpa). Using these assays, we found that many candidate genes are required for basal and/or NB-LRR mediated immunity. These include genes encoding proteins involved in transcriptional control, auxin response, lipid metabolism and protein translational control. These studies have allowed us to identify new actors in plant defense pathways and future studies will focus on how their translation is regulated by NB-LRR signaling and how their protein products contribute to plant defenses.

Abstract Number: P17-491
Session Type: Poster