Identifying the receptor of Phytophthora infestans apoplastic effector SCR74 from wild potato 
X. LIN (1) (1) Wageningen UR, China

The potato (Solanum tuberosum L.) is one of the three most consumed crops worldwide. The most devastating disease of potato is late blight, which is caused by the oomycete Phytophthora infestans. Traditional disease resistance breeding that exploits cytoplasmic resistance genes (R genes) has been of limited success, as P. infestans has a remarkable capacity to rapidly adapt to resistant plants. Another, yet unexploited layer of immunity occurs at the surface of plant cells, and is typically mediated by receptor-like proteins (RLP) and receptor-like kinases (RLK) To obtain novel potato pattern recognition receptors (PRRs), we are deploying an effectoromics approach [1]. SCR74, a small cysteine-rich protein of a highly polymorphic gene family was subjected to functional screens on wild potato species. Solanum microdontum ssp. gigantophyllum exhibited specific responses to SCR74 [2]. To accelerate gene mapping and cloning, we deployed an RLK/ RLP enrichment sequencing in the segregating populations [3]. The putative gene that triggers SCR74 response is mapped to a 100kb interval on the reference genome of potato, and might likely be identified soon. Ultimately, we aim to pyramid diverse types of immune receptors in cultivated potato to maximize the potential of generating a broader and potentially more durable resistance to Phytophthora. 

Abstract Number: P17-560
Session Type: Poster