Genome-wide comparative analyses and evolutionary history of nucleotide-binding leucine-rich repeat (NLR) gene family among Solanaceae plants
E. SEO (1), S. Yeom (2), H. Lee (3), D. Choi (3) (1) Seoul National University, South Korea; (2) Gyeongsang National University, South Korea; (3) Seoul National University, South Korea

Plants have evolved elaborate innate immune systems against invading pathogens, such as bacteria, viruses, fungi, and oomycetes. Among them, intracellular immune receptors known as nucleotide-binding leucine-rich repeat (NLR) play important roles in effector-triggered immunity (ETI) regarding to plant defense. Here, we performed genome-wide identification and classification analyses of NLR-coding sequences and comparative analysis with Solanaceae plants. We identified 267, 443, and 755 genes having NB domain in the genome of tomato, potato, and pepper, respectively. Phylogenetic and clustering analyses revealed that they were classified into the 14 subgroups including 1 TNL and 13 CNL types. Interestingly, the numbers of genes in CNL-G1 and CNL-G2 subgroup were highly increased compared to other subgroup showing a large portion of NLR in pepper, even though the numbers of total predicted proteins were similar among the genomes. In addition, 623 out of 755 NLR pepper proteins were physically mapped onto 12 chromosome pseudomolecules showing many tandem array in the specific chromosome. Comparative analysis revealed distinct duplication patterns within pepper or among Solanaceae plants suggesting species-specific gene-duplication events after speciation. Genome-wide identification of NLR family and insights of their evolutionary history in Solanaceae could provide important resources for identification and characterization of R genes for breeding disease resistance crops.

Abstract Number: P17-605
Session Type: Poster