Characterization of the Xa10 paralog genes in rice cultivar Nipponbare for disease resistance to Xantomonas oryzae pv oryzae
Z. YIN (1), J. Wang (2), X. Zeng (2), D. Tian (2), K. Gu (2), X. Yang (2), L. Wang (2), K. Ong (2) (1) Temasek Life Sciences Laboratory, Singapore; (2) Temasek Life Sciences Laboratory, Singapore

Rice bacterial blight, caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the most important bacterial diseases prevalent throughout the major rice-growing regions. The utilization of host resistance is the most effective and economical way to control the disease. The rice resistance (R) gene Xa10 confers race-specific disease resistance to incompatible Xoo strains that produces the corresponding transcription activator-like (TAL) type III effector AvrXa10. The AvrXa10-depended Xa10 induction triggers hypersensitive response (HR), a form of programed cell death in plants. Rice cultivar Nipponbare, which is susceptible to most of the Xoo strains tested, carries two Xa10 paralog genes, Xa10P1 and Xa10P2, which are allelic to the Xa10 locus. Here we report the characterization of the two Xa10 paralog genes by employing forward genetics with dTALE and transgenic approaches and reverse genetics using TALEN and CRIPR/Cas-9 technology. Our initial results demonstrated that both Xa10P1 and Xa10P2 are R genes for bacterial blight resistance when induced by dTALE. Further molecular, cellular and biochemical studies indicated that Xa10P1 and Xa10P2 gene products function through similar mechanism to that of XA10. XA10, XA10P1 and XA10P2 belong to a group of R proteins that localizes to the ER membrane and trigger cell death by inducing Ca2+ depletion in the ER lumen.

Abstract Number: P17-643
Session Type: Poster