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Unraveling Pseudomonas syringae pv. actinidiae intra and inter-species signalling
G. BURIANI (1), L. Fiorentini (2), V. Venturi (3), F. Spinelli (2) (1) University of Bologna, Italy; (2) University of Bologna, Italy; (3) ICGEB, Italy
The bacterial communication is a complex phenomenon that involves a heterogeneous class of compounds and effect different physiological features. Pseudomonas syringae pv. actinidiae (Psa), the pathogen responsible of the most sever kiwifruit disease, although lacking a canonical signaling system, presents at least 3 signal receptors. The presented study aimed to clarify: 1. The response of Psa to the classical cell density signals or the presence of a still not characterized cell density signaling system 2. The presence of an interspecific signaling system among Psa an other associated kiwifruit pathogens. Biofilm formation, motility and virulence were studied to evaluate the presence of putative signaling systems. A bioinformatic analysis of Psa genome, allowed to identify a putative transcriptional regulator which could be involved in signaling transduction. This gene, together with biofilm, motility and virulence related genes were analyzed by QPCR in Psa samples derived from different culture conditions (high/ low density, addition of exogenous AHLs, co-colture with other bacteria). The studied phenotypes seem to be slightly influenced by the cell density. On the other hand, the addition of C6-OH-homoserinelactones and C8-OH-homoserinelactones affected Psa behavior according the signal dose. Finally, also the presence of Pss and Pv influence Psa response thus suggesting an inter-species signalling system in the epiphytic bacterial community of kiwifruit
Abstract Number:
P4-88
Session Type:
Poster
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