Antimicrobial mechanism of peptides derived from Trichoderma
S. LIU (1), H. Lin (2), M. Hsieh (1), K. Peng (2) (1) Department of Molecular Biotechnology, Da-Yeh University, Taiwan; (2) Department of Life Science, National Dong Hwa University, Taiwan

Due to abuse of antibiotics, multi-drug resistance microorganisms have increased. Instead of traditional chemical compound, antimicrobial peptide has arisen to be one of the candidates of new generation antibiotic. L-amino acid oxidase from Trichoderma harzianum (Th-LAAO) was demonstrated to inhibit bacteria and fungi growth via mitochondria-dependent apoptosis and cell leakage. Therefore, we further investigate the potential antimicrobial capabilities of synthesized N-terminal sequence (TLN) of Th-LAAO. In this research, the antimicrobial activity of 21-residues TLN was evaluated while against 6 bacteria: Acidovorax avenae subsp. Citrulli (Aac), Escherichia coli BCRC10675 (EC), Pectobacterium carotovorum subsp. Carotovorum (Pcc), Pantoea stewartii subsp. Stewartii (Pss), Ralstonia solanacearum (Rs), Staphylococcus aureus BCRC10780 (Sa). As a result, the cell leakage of two bacteria was induced significantly by the synthesized N-terminal sequence (TLN) of Th-LAAO. The potential of using TLN as antimicrobial treatment is high and the underlining mechanism is under investigation.

Abstract Number: P7-187
Session Type: Poster