An endophytic bacterium, Bacillus velezensis YC7010 induces systemic resistance against brown planthopper in rice
Y. CHUNG (1), H. Rhasid (1), A. Khan (1), M. Hossain (2), J. Hwang (1) (1) Gyeongsang National University, South Korea; (2) Regional Agricultural Research Station, Bangladesh

Brown planthopper (BPH; Nilaparvata lugens Stål) is one of the most serious insect pests which reduce rice yield remarkably in many rice growing areas. Some plant growth-promoting rhizobacteria induce systemic resistance against herbivorous insects. Here we show that root drenching of rice seedlings with Bacillus velezensis YC7010 induced systemic resistance (ISR) against brown planthopper. On the basis of next generation deep-sequencing transcriptome analysis, ISR induced by YC7010 in rice against brown planthopper was demonstrated to be through both salicylic acid (SA) and jasmonic acid (JA) dependent pathways. Expression of JA biosynthesis genes such as lipoxygenase, jacalin-like lectin and SA dependent gene, phenylalanine ammonia-lyase were increased in YC7010 treated rice plants. Some genes related to receptor kinase, Ca2+ signaling, stress signaling, protein modification and transcription factors were increased in YC7010- inoculated and control plants by the infestation of brown planthopper. On the other hand, several genes related to JA biosynthesis, jacalin-like lectin, peroxidase biosynthesis, photosynthesis, carbohydrate synthesis, lipid metabolism and cell wall structural protein and secondary metabolism were up-regulated in BPH infested plants treated with YC7010 compared to the untreated BPH infested plants. This study shows for the first time that the higher expression of genes related to peroxidase biosynthesis, cell wall structural protein, cellulose synthase, phenyl-propanoid and terpenoid biosynthetic pathways leads to the strengthening physical barriers in rice associated with the SA and JA dependent resistance against BPH mediated by YC7010.

Abstract Number: P9-247
Session Type: Poster