The role of a glycosylphosphatidylinositol-anchored protein in plant immunity
Q. SHEN (1), H. Pan (1), D. Tang (1) (1) Institute of Genetics and Development Biology, Chinese Academy of Sciences, China

Plants use membrane-localized pattern recognition receptors (PRRs) to detect and respond to pathogen invasion by recognizing pathogen-associated molecular patterns (PAMPs) and activating downstream immune responses. Arabidopsis thaliana ENHANCED DISEASE RESISTANCE 1 (EDR1), a Raf-like MAPK kinase kinase plays a negative role in plant immunity. In a forward genetic screen for suppressors of edr1, we identified that EDOS2 (ENHANCED DISEASE RESISTANCE ONE SUPPRESSOR 2), contributes to edr1-activated resistance. The edos2 mutants show enhanced susceptibility to various virulent pathogens, indicating that EDOS2 has an important role in plant immunity. EDOS2 encodes a glycosylphosphatidylinositol (GPI)-anchored protein, and localizes in the plasma membrane. We found that EDOS2 constitutively associates with the PAMP receptor FLAGELLIN SENSING 2 (FLS2) and forms a complex with BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED RECEPTOR KINASE 1 (BAK1) in a ligand-dependent manner, indicating that EDOS2 functions as a key component of PAMP-recognition immune complexes. Moreover, EDOS2 is required for downstream innate immunity responses, including ligand-induced phosphorylation of BOTRYTIS-INDUCED KINASE 1 (BIK1) and production of reactive oxygen species. Taking these results together, our study indicates that EDOS2 associates with PAMP receptor and plays an important role in plant innate immunity.

Abstract Number: C24-2
Session Type: Concurrent