Rice stripe virus overcomes NbREM-mediated inhibition of movement through interference of S-acylation
X. ZHOU (1), S. Fu (2), Y. Xu (2), C. Li (2) (1) Institute of Plant Protection, Chinese Academy of Agricultural Sciences, China; (2) Zhejiang university, China

Rice stripe virus (RSV) is a single-stranded RNA virus belonging to the genus Tenuivirus and is transmitted by the small brown planthopper, Laodelphax striatellus. RSV mainly infects rice plants and causes dramatic yield losses in China. RSV can also mechanically infect Nicotiana benthamiana, which provides us a convenient platform to study RSV-plant interaction. An iTRAQ-based proteomic level survey of RSV infection responses in experimental host plant N. benthamiana was performed. Total 142 differentiated accumulated proteins were identified. Remorin (NbREM), a plant specific plasma membrane microdomain marker, is down-regulated by RSV infection. Deletion of NbREM using CRISPR/CAS9 in N. benthamiana enhances RSV infection, while overexpression of NbREM attenuates RSV infection in N. benthamiana. We also demonstrated that S-acylation is required for NbREM to correctly target to plasma membrane, while S-acylation deficient NbREM retained its location in the ER and cytoplasm. Furthermore, we found that S-acylation of NbREM contributes to the protein stability, and in the absence of S-acylation, NbREM entered a degradation pathway. In addition, we demonstrated that RSV and its movement protein triggered NbREM degradation though autophagy pathway.

Abstract Number: S2-2
Session Type: Special Session